Fletcher’s hopes of becoming the club’s all time games record holder have been put on hold with the veteran currently sitting equal with Simon Madden on 378 games.
Football Operations Manager Steve Alessio said Fletcher suffered the injury at training last week.
“Dustin last weekend hurt his hamstring during training, we tested it out during the week but he is going to miss this weekend’s game with a hamstring injury,” Alessio said.
“It is disappointing for Dustin and us that he won’t be out there for his 379th game.”
Groin issues have meant Fletcher has played only 13 matches in this his 21st season.
The 38-year-old has recently indicated his desire to play on next season but a decision will be made in consultation with the club over the coming weeks.
